Incretins and Metabolism

This study is currently recruiting participants.
Verified January 2013 by Rigshospitalet, Denmark
Sponsor:
Information provided by (Responsible Party):
Thomas Solomon, Rigshospitalet, Denmark
ClinicalTrials.gov Identifier:
NCT01607944
First received: May 23, 2012
Last updated: January 7, 2013
Last verified: January 2013

May 23, 2012
January 7, 2013
April 2012
April 2014   (final data collection date for primary outcome measure)
Glucose turnover [ Time Frame: 0, 1, 2, 3, 4 hours ] [ Designated as safety issue: No ]
Rates of appearance and disappearance (g/min) will be measured by examining [6,6-2H2]glucose enrichment in plasma.
Same as current
Complete list of historical versions of study NCT01607944 on ClinicalTrials.gov Archive Site
  • Blood flow and flow-mediated dilation [ Time Frame: 0, 1, 2, 3, 4 hours ] [ Designated as safety issue: No ]
  • Palmitate turnover and oxidation [ Time Frame: 0, 1, 2, 3, 4 hours ] [ Designated as safety issue: No ]
    Rates of appearance and disappearance (g/min) will be measured by examining [U13C]palmitate enrichment in plasma. Rate of oxidation (g/min) will also be measured by examining 13C incorporation into CO2 in expired breath.
Same as current
Not Provided
Not Provided
 
Incretins and Metabolism
The Extrapancreatic Metabolic Effects of Incretin Hormones

Incretin hormones (GLP-1 and GIP) have insulin secretory effects on the pancreas that are glucose dependent. Extrapancreatic effects of incretin hormones are reported, however the glucose dependency of these effects have not been examined. In type 2 diabetes, pancreatic endocrine function and incretin metabolism are impaired. The investigators hypothesize that extrapancreatic effects of incretin hormones are glucose depedent and dysregulated in subjects with type 2 diabetes.

Healthy control subjects and type 2 diabetics will undergo pancreatic clamps. In brief, somatostatin will be infused to inhibit pancreatic endocrine function and basal levels of insulin, glucagon, and growth hormone will be replace via infusion. Metabolic flux will be studied during euglycemic and hyperglycemic stages of the pancreatic clamp. Each subject will undergo 3 trials involving the co-infusion of either saline(Control Trial), GLP-1, or GIP. Glucose metabolism will be assessed using 13C-glucose stable isotope methodology combined with indirect calorimetry and expired breath isotope ratio analysis. Blood flow and flow-mediated dilation will be measured using ultrasound Doppler. Skeletal muscle and abdominal adipose biopsies will be taken to examine intracellular signalling.

Not Provided
Observational
Observational Model: Case Control
Time Perspective: Cross-Sectional
Not Provided
Not Provided
Non-Probability Sample

Local community volunteers

Type 2 Diabetes
Not Provided
  • Normal Glucose Tolerance
  • Type 2 Diabetes
Not Provided

*   Includes publications given by the data provider as well as publications identified by ClinicalTrials.gov Identifier (NCT Number) in Medline.
 
Recruiting
24
Not Provided
April 2014   (final data collection date for primary outcome measure)

Inclusion Criteria:

  • age 18-60 years
  • BMI 18-35 kg/m2
  • NGT or T2DM as classified by ADA criteria

Exclusion Criteria:

  • Insulin dependency
  • Smokers
  • History of or presentation with cardiovascular disease, cancer, and chronic hematological, renal, hepatic, pulmonary disease
  • Weight loss (>2 kg in previous 6 months)
  • Physical activity (>150 mins/week)
Male
18 Years to 60 Years
Yes
Contact: Thomas P Solomon, PhD thomas.solomon@inflammation-metabolism.dk
Contact: Kristian Karstoft, MD
Denmark
 
NCT01607944
NNF2012
No
Thomas Solomon, Rigshospitalet, Denmark
Rigshospitalet, Denmark
Not Provided
Principal Investigator: Thomas P Solomon, PhD Rigshospitalet, Denmark
Rigshospitalet, Denmark
January 2013

ICMJE     Data element required by the International Committee of Medical Journal Editors and the World Health Organization ICTRP