miRNA Machinery in Melanoma, Melanoma Metastases and Benign Melanocytic Naevi
MicroRNAs (miRNAs) are very small endogenous RNA molecules about 22-25 nucleotides in length, capable of post-transcriptional gene regulation. miRNAs bind to their target messenger RNAs (mRNAs), leading to cleavage or suppression of target mRNA translation based on the degree of complementarity. miRNAs have recently been shown to play pivotal roles in diverse developmental and cellular processes and linked to a variety of skin diseases and cancers. In the present study, the investigators examines the expression profiles of miRNA machinery components such as miRNA maturation and transport factors, microprocessor complex and RISC subunits in cutaneous melanoma, cutaneous melanoma metastases and benign melanocytic nevi.
|Study Design:||Observational Model: Cohort
Time Perspective: Prospective
|Official Title:||The miRNA Machinery in Melanoma, Melanoma Metastases and Benign Melanocytic Naevi|
|Study Start Date:||December 2008|
|Study Completion Date:||September 2011|
|Primary Completion Date:||September 2011 (Final data collection date for primary outcome measure)|
|Cutaneous Melanoma Metastases|
|Benign Melanocytic Nevi|
Patients with cutaneous melanoma, cutaneous melanoma metastases and benign melanocytic nevi were included in the study. Punch biopsies were harvested from the center of the tumors. The miRNA machinery components were detected by quantitative real-time reverse transcriptase polymerase chain reaction.
|Department of Dermatology, Venereology and Allergology, Ruhr-University Bochum|
|Bochum, NRW, Germany, 44791|
|Study Director:||Falk G Bechara, PD Dr.||Department of Dermatology, Venereology and Allergology, Ruhr-University Bochum|